The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...
To many working biologists, DNA-modifying enzymes are tools, not unlike a pair of molecule-sized scissors. To Dr. Barry Stoddard, a professor in the Basic Sciences division at Fred Hutch, these ...
The origin of SARS-CoV-2 remains unresolved. In a non-peer-reviewed preprint published on bioRxiv on Oct. 20, 2022 three authors present analyses that, according to their interpretation, suggest a ...
Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...
Glenn, T. C., Waller, D. R., and Braun, Michael J. 1994. "Increasing proportions of uracil in DNA substrates increases inhibition of restriction enzyme digests ...
CRISPR—Clustered Regularly Interspaced Short Palindromic Repeats—is the microbial world’s answer to adaptive immunity. Bacteria don’t generate antibodies when they are invaded by a pathogen and then ...
Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...
The origin of SARS-CoV-2 remains unresolved. In a non-peer-reviewed preprint published on bioRxiv on Oct. 20, 2022 three authors present analyses that, according to their interpretation, suggest a ...